Gyanu Lamichhane, PhD
Associate Professor, Department of Medicine, Graduate Program in Pathobiology, Graduate Program in Cellular & Molecular Medicine.
I am a scientist with an interest in understanding fundamental mechanisms used by Mycobacterium tuberculosis to survive, grow and cause disease. Although our lab uses genetic and biochemical approaches to study this organism, we pursue questions irrespective of the expertise required to answer those questions. This means we will learn approaches/techniques that may be outside of our technical comfort zone. My laboratory undertakes basic science studies and simultaneously seeks to translate them for use in clinic.
The two major questions we are pursuing are: What are the essential components of the peptidoglycan layer and how is the physiology of this layer maintained? Please note that the peptidoglycan layer is an essential ‘organelle’. In 2007, we accidentally identified a transpeptidase that generates mDAP-mDAP or 3→3 transpeptide linkages in the peptidoglycan layer of M. tuberculosis. We are now seeking to comprehensively understand the biology of the peptidoglycan layer. The ultimate aim is to find effective ways to destroy or inhibit biosynthesis of this layer. What non-coding RNAs exist in M. tuberculosis and what is their relevance to physiology and virulence of this pathogen? In addition to rRNAs, tRNAs and tmRNA, M. tuberculosis generates a number of other non-coding RNAs. We study these RNAs.