Microbiology, Immunology, Animal Modeling & Imaging

Microbiology, Immunology, Animal Models and Imaging (MIAMI) Core provides decades of combined research and mentoring expertise, as well as access to cutting-edge laboratory technologies to enable training opportunities in and support for TB research in microbiology, molecular genetics, and biosafety level-3 (BSL3) handling of Mycobacterium tuberculosis; immunologic tools to assess host responses to TB infection and diseases; and multiple clinically-relevant animal models of TB , alongside the capacity to conduct in vivo imaging with CT, PET, and SPECT.

Our Services

Standardization of experimental protocols
Support for animal models, cytokine analysis, scRNA-seq, and flow cytometry
Training and services in advanced whole-body live animal imaging (CET, PET, SPECT, new MRI)
Standardized methods for data sharing, analysis and training
Analysis of high-throughput (e.g., transcriptomics, metabolomics) and imaging data
Hands-on training for animal-related procedures/endpoints

Featured Publications

Immunotherapy targeting drug-tolerant Mycobacterium tuberculosis persisters accelerates tuberculosis cure in preclinical models (Karanika et al, 2026)

Congratulations to Dr. Styliani Karanika, who is a JHU TRAC ESI, Mentee, and Developmental Awardee on this publication in the Journal of Clinical Investigation! The open-access full ...

Structure-based virtual screening and in vitro validation of inhibitors of cyclic dinucleotide phosphodiesterases ENPP1 and CdnP

Rohilla A, Singh AK, Koleske B, Srikrishna G, Bishai WR. Structure-based virtual screening and in vitro validation of inhibitors of cyclic dinucleotide phosphodiesterases ENPP1 and CdnP. Microbiol Spectr. ...

Linezolid does not improve bactericidal activity of rifampin-containing first-line regimens in animal models of TB meningitis

Tucker EW, Ruiz-Bedoya CA, Mota F, Erice C, Kim J, de Jesus P, Jahdav R, Bahr M, Flavahan K, Chen X, Peloquin CA, Freundlich JS, ...

Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosis

Parveen, S., Shen, J., Lun, S. et al. Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosis. Nat Commun 14, 7427 (2023). https://doi.org/10.1038/s41467-023-43304-0

The Mycobacterium tuberculosis genome at 25 years: lessons and lingering questions

Koleske BN, Jacobs WR Jr, Bishai WR. The Mycobacterium tuberculosis genome at 25 years: lessons and lingering questions. J Clin Invest. 2023;133(19):e173156. Published 2023 Oct 2. ...

HIV/Tuberculosis Coinfection in Pregnancy and the Postpartum Period

Yilma A, Bailey H, Karakousis PC, Karanika S. HIV/Tuberculosis Coinfection in Pregnancy and the Postpartum Period. J Clin Med. 2023;12(19):6302. Published 2023 Sep 29. doi:10.3390/jcm12196302

Sex and Gender Differences in Tuberculosis Pathogenesis and Treatment Outcomes

Dabitao D, Bishai WR. Sex and Gender Differences in Tuberculosis Pathogenesis and Treatment Outcomes. Curr Top Microbiol Immunol. 2023;441:139-183. doi: 10.1007/978-3-031-35139-6_6. PMID: 37695428.

Functional Whole Genome Screen of Nutrient-Starved Mycobacterium tuberculosis Identifies Genes Involved in Rifampin Tolerance

Matern WM, Harris HT, Danchik C, et al. Functional Whole Genome Screen of Nutrient-Starved Mycobacterium tuberculosis Identifies Genes Involved in Rifampin Tolerance. Microorganisms. 2023;11(9):2269. Published 2023 Sep 9. ...

Comparative immune responses to Mycobacterium tuberculosis in people with latent infection or sterilizing protection

Jalbert E, Liu C, Mave V, Lang N, Kagal A, Valvi C, Paradkar M, Gupte N, Lokhande R, Bharadwaj R, Kulkarni V, Gupta A, Weinberg ...

Differentially culturable tubercle bacteria as a measure of tuberculosis treatment response

Peters JS, McIvor A, Papadopoulos AO, Masangana T, Gordhan BG, Waja Z, Otwombe K, Letutu M, Kamariza M, Sterling TR, Bertozzi CR, Martinson NA, Kana ...

Dynamic 18F-Pretomanid PET imaging in animal models of TB meningitis and human studies

Mota F, Ruiz-Bedoya CA, Tucker EW, Holt DP, De Jesus P, Lodge MA, Erice C, Chen X, Bahr M, Flavahan K, Kim J, Brosnan MK, ...

Transcriptional Analysis for Tuberculosis in Pregnant Women From the PRegnancy Associated Changes In Tuberculosis Immunology (PRACHITi) Study

Mathad JS, Queiroz ATL, Bhosale R, Alexander M, Naik S, Kulkarni V, Andrade BB, Gupta A. Transcriptional Analysis for Tuberculosis in Pregnant Women From the ...

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Featured Publications

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